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KMID : 0900919980220030293
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Korean journal of Animal Reproduction
1998 Volume.22 No. 3 p.293 ~ p.300
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Effect of Thiol Compounds on the Blastocyst Formation of In Vitro Matured and Fertilized Bovine Embryos
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Uhm S.-J.
Lee Hoon-Taek
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Abstract
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The objective of this study was to determine effects of beta-mercaptoethanol (beta-ME) and cyst-eine (CYS) on the development of bovine em-bryos obtained from in vitro matured and fertil-ized oocytes. Cumulus-oocyte-complexes (COC-s) were matured in micro-drop of TCM-199 medium containing 10% FBS, 17beta-Estradiol and FSH-p under paraffin oil at 39^{circ}C for 24 hrs. The fertilization of COC were induced in Fert-TALP medium supplemented with PHE, heparin, BSA and then the fertilized oocytes were cultured in CR1aa medium for 24 hrs. To investigate the effects of the agents on the development of the embryos, the embryos developed to the late 2-cell stage were cultured in the media with and without beta-ME, CYS for 9 days. In experiment 1, to select appropriate concentration of beta-ME and CYS during whole culture period (9 days), various concentrations of beta-ME and CYS were add ded to the CR1aa medium. Addition of 25TEX>muM of beta-ME and O.1mM of CYS to the culture medium 1 increase the incidence of embryos developed to the blastocyst. In experiment 2, we evaluated the effects of 25muM of beta-ME and O.1mM of CYS addition on the blastocyst formation when emb bryos at different stages were exposed to 25muM beta-ME and O.1mM of CYS. beta-ME and CYS enhanced in vitro development of embryos to the blastocyst stage. The effect was greater in 8-ceII to morula embryos than in embryos fewer than 2-cells at the initiation of treatment. These results suggested that the addition of 25muM B-ME and O.1mM cysteine enhanced development to the blastocyst and hatching stage of in vitro derived bovine embryos, also addition of beta-ME and cysteine were effective later stage embryo than early embryo development.
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KEYWORD
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Thiol compound, beta-Mercaptoethanol, Cysteine
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